5LHC

The structure of D456A mutant of Nt.BspD6I nicking endonuclease at 0.24 nm resolution .

Replaces:  4WL5

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Nicking endonuclease N.BspD6I	polymer	610	71658.1	2	UniProt (Q8GCA3) Pfam (PF09491)	Bacillus sp.
2	C, F, G, H (A, B)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
3	D, E, I, J (A, B)	PHOSPHATE ION	non-polymer		95.0	4	Chemie (PO4)
4	K, L (A, B)	water	water		18.0	173	Chemie (HOH)

Sequence modifications

A, B: 1 - 604 (UniProt: Q8GCA3)

PDB	External Database	Details
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ala 456	Asp 456	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	143316.1
Non-Polymers*	Number of molecules	8
	Total formula weight	748.3
All*	Total formula weight	144064.4

*Water molecules are not included.