5LHC
The structure of D456A mutant of Nt.BspD6I nicking endonuclease at 0.24 nm resolution .
Replaces: 4WL5Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Nicking endonuclease N.BspD6I | polymer | 610 | 71658.1 | 2 | UniProt (Q8GCA3) Pfam (PF09491) In PDB | Bacillus sp. | |
2 | A, B | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
3 | A, B | PHOSPHATE ION | non-polymer | 95.0 | 4 | Chemie (PO4) | |||
4 | water | water | 18.0 | 173 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 604 (UniProt: Q8GCA3)
PDB | External Database | Details |
---|---|---|
His -5 | - | expression tag |
His -4 | - | expression tag |
His -3 | - | expression tag |
His -2 | - | expression tag |
His -1 | - | expression tag |
His 0 | - | expression tag |
Ala 456 | Asp 456 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 143316.1 | |
Non-Polymers* | Number of molecules | 8 |
Total formula weight | 748.3 | |
All* | Total formula weight | 144064.4 |