5LGD
The CIDRa domain from MCvar1 PfEMP1 bound to CD36
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.826, 40.726, 138.836 |
| Unit cell angles | 90.00, 114.85, 90.00 |
Refinement procedure
| Resolution | 62.990 - 2.070 |
| R-factor | 0.2145 |
| Rwork | 0.212 |
| R-free | 0.25260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f7b |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.190 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 63.000 |
| High resolution limit [Å] | 2.070 |
| Number of reflections | 40626 |
| <I/σ(I)> | 8.2 |
| Completeness [%] | 99.8 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M NaCl, 20% (w/v) PEG6000, 0.1 M Tris, pH 8.0 |
