5LG3
X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) in complex with chlorpromazine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-06-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 105.200, 266.650, 111.040 |
| Unit cell angles | 90.00, 107.71, 90.00 |
Refinement procedure
| Resolution | 44.746 - 3.567 |
| R-factor | 0.2217 |
| Rwork | 0.220 |
| R-free | 0.25310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a97 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.933 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.160 | |
| High resolution limit [Å] | 3.400 | |
| Rmerge | 0.093 | 0.037 |
| Number of reflections | 67372 | |
| <I/σ(I)> | 14.8 | |
| Completeness [%] | 98.9 | |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 200 mM ammonium sulfate 50 mM ADA (N-(2-acetamido)iminodiacetic acid) pH 6.5 9-12 % PEG 4000 |
