5LFR

Crystal structure of glycosylated Myelin-associated glycoprotein (MAG) Ig1-3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-27
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97599
Spacegroup name	P 1
Unit cell lengths	43.060, 60.400, 79.220
Unit cell angles	72.70, 86.71, 83.01

Refinement procedure

Resolution	42.729 - 2.120
R-factor	0.226
Rwork	0.224
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1url 4frw 1cs6
RMSD bond length	0.003
RMSD bond angle	0.700
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.730	2.190
High resolution limit [Å]	2.120	2.120
Rmerge	0.064	1.118
Number of reflections	42931
<I/σ(I)>	8.8	0.9
Completeness [%]	96.2	95.7
Redundancy	3.6	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.05 M tri-sodium citrate, 1.2 M ammonium sulfate, 3 % (w/v) isopropanol