5LF5
Myelin-associated glycoprotein (MAG) deglycosylated full extracellular domain with co-purified ligand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-08-22 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.99998 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 278.868, 278.868, 62.520 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 52.701 - 3.800 |
R-factor | 0.2005 |
Rwork | 0.199 |
R-free | 0.22810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1url 4frw 1cs6 3p3y 2yd6 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.397 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.700 | 4.030 |
High resolution limit [Å] | 3.800 | 3.800 |
Rmerge | 0.233 | 1.685 |
Number of reflections | 27623 | |
<I/σ(I)> | 9.3 | 1.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.6 | 9.7 |
CC(1/2) | 0.997 | 0.565 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 100 mM NaCl, 20 mM Tris/HCl pH 7.0, 7.7 % PEG 4000 (w/v) |