5LDB
Crystal Structure of Polyphosphate Kinase from Meiothermus ruber bound to ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-14 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.000010 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 165.619, 165.619, 95.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.750 - 2.300 |
| R-factor | 0.179 |
| Rwork | 0.177 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lc9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.000 |
| Data scaling software | Aimless (0.5.25) |
| Phasing software | MOLREP |
| Refinement software | BUSTER-TNT (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.750 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.470 | 4.798 |
| Number of reflections | 59190 | |
| <I/σ(I)> | 10.3 | 1.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 27 | 28.2 |
| CC(1/2) | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | PEG3350, Li2SO4 |
