5LCV

Structural basis of Zika and Dengue virus potent antibody cross-neutralization

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-23
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98013
Spacegroup name	C 2 2 21
Unit cell lengths	204.288, 207.317, 124.576
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.640
R-factor	0.2242
Rwork	0.224
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uta 4UT7 and 4UTB
RMSD bond length	0.007
RMSD bond angle	1.000
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.640
Rmerge	0.500
Number of reflections	77483
<I/σ(I)>	5.1
Completeness [%]	99.8
Redundancy	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	3.5M Na Formate 0.1M Tris pH 8.5