5LCV
Structural basis of Zika and Dengue virus potent antibody cross-neutralization
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-23 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98013 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 204.288, 207.317, 124.576 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.640 |
| R-factor | 0.2242 |
| Rwork | 0.224 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uta 4UT7 and 4UTB |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 2.640 |
| Rmerge | 0.500 |
| Number of reflections | 77483 |
| <I/σ(I)> | 5.1 |
| Completeness [%] | 99.8 |
| Redundancy | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 3.5M Na Formate 0.1M Tris pH 8.5 |
