5LAX
Crystal structure of HLA_DRB1*04:01 in complex with alpha-enolase peptide 26-40
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.176, 73.450, 144.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.560 - 2.600 |
R-factor | 0.19491 |
Rwork | 0.191 |
R-free | 0.26965 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5jlz |
RMSD bond length | 0.013 |
RMSD bond angle | 1.614 |
Data reduction software | XDS (13.14.0) |
Data scaling software | XDS (13.14.0) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.200 | 2.750 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 138347 | |
<I/σ(I)> | 7.8 | 1.8 |
Completeness [%] | 99.6 | 98.8 |
Redundancy | 5.9 | 6 |
CC(1/2) | 0.989 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 100 mM sodium malonate, pH 4.0, 12 to 18 percent (vol/vol) PEG3350 |