5LA9
HIF prolyl hydroxylase 2 (PHD2-R281C/V314C) cross-linked to HIF-1alpha NODD-L397C/D412C and N-oxalylglycine (NOG) (complex-2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-04 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 88.467, 97.329, 71.001 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.129 - 2.813 |
R-factor | 0.2525 |
Rwork | 0.251 |
R-free | 0.27850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hqr |
RMSD bond length | 0.003 |
RMSD bond angle | 0.612 |
Data reduction software | HKL-2000 (706E) |
Data scaling software | HKL-2000 (706E) |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.129 | 2.900 |
High resolution limit [Å] | 2.810 | 2.810 |
Rmerge | 0.245 | 1.250 |
Number of reflections | 15152 | |
<I/σ(I)> | 4.7769 | 1.7378 |
Completeness [%] | 98.5 | 99.3 |
Redundancy | 3.4 | 3.5 |
CC(1/2) | 0.993 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES pH 7.5, 2.0 M ammonium sulfate |