5LA9
HIF prolyl hydroxylase 2 (PHD2-R281C/V314C) cross-linked to HIF-1alpha NODD-L397C/D412C and N-oxalylglycine (NOG) (complex-2)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Egl nine homolog 1 | polymer | 252 | 27928.7 | 2 | UniProt (Q9GZT9) Pfam (PF13640) In PDB | Homo sapiens (Human) | Hypoxia-inducible factor prolyl hydroxylase 2,HPH-2,Prolyl hydroxylase domain-containing protein 2,PHD2,SM-20 |
2 | C, D | Hypoxia-inducible factor 1-alpha | polymer | 19 | 1895.2 | 2 | UniProt (Q16665) In PDB | Homo sapiens | HIF1-alpha,ARNT-interacting protein,Basic-helix-loop-helix-PAS protein MOP1,Class E basic helix-loop-helix protein 78,bHLHe78,Member of PAS protein 1,PAS domain-containing protein 8 |
3 | A, B | MANGANESE (II) ION | non-polymer | 54.9 | 2 | Chemie (MN) | |||
4 | A, B | N-OXALYLGLYCINE | non-polymer | 147.1 | 2 | Chemie (OGA) | |||
5 | A | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
6 | water | water | 18.0 | 74 | Chemie (HOH) |
Sequence modifications
A, B: 181 - 426 (UniProt: Q9GZT9)
C, D: 395 - 413 (UniProt: Q16665)
PDB | External Database | Details |
---|---|---|
Gly 175 | - | expression tag |
Ser 176 | - | expression tag |
His 177 | - | expression tag |
Met 178 | - | expression tag |
Ala 179 | - | expression tag |
Ser 180 | - | expression tag |
Ala 201 | Cys 201 | engineered mutation |
Cys 281 | Arg 281 | engineered mutation |
Cys 314 | Val 314 | engineered mutation |
Ala 398 | Arg 398 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Cys 397 | Leu 397 | engineered mutation |
Cys 412 | Asp 412 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 59647.9 | |
Non-Polymers* | Number of molecules | 5 |
Total formula weight | 500.1 | |
All* | Total formula weight | 60148.0 |