5LA4
Crystal structure of apo human proheparanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.501, 88.061, 120.497 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.580 - 1.900 |
| R-factor | 0.18743 |
| Rwork | 0.185 |
| R-free | 0.22729 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5e8m |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.572 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.580 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.038 | 0.397 |
| Number of reflections | 43065 | |
| <I/σ(I)> | 28 | 5.3 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 7.9 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 0.1 M Succinate pH 7.0 17% PEG3350 1:250 dilution seed stock - Seed stock grown in 0.2M Ammonium Nitrate, 20% PEG3350 |
