5L9X
Human DNA polymerase eta-DNA ternary complex: reaction with 10 mM Mn2+ for 60s
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-03 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 |
Unit cell lengths | 98.290, 98.290, 82.050 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.942 - 1.900 |
R-factor | 0.1683 |
Rwork | 0.165 |
R-free | 0.21430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ecv |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.950 | |
High resolution limit [Å] | 1.900 | 8.500 | 1.900 |
Rmerge | 0.081 | 0.016 | 0.684 |
Number of reflections | 35503 | ||
<I/σ(I)> | 20.9 | 90.3 | 3.08 |
Completeness [%] | 99.9 | 91.6 | 99.8 |
Redundancy | 6.9 | ||
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6 | 298 | 17% PEG 2000MME, 0.1 M MES |