5L9V

HIF prolyl hydroxylase 2 (PHD2-R281C/P317C) cross-linked to HIF-1alpha NODD-L397C/D412C and N-oxalylglycine (NOG) (complex-1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	43.807, 73.088, 70.407
Unit cell angles	90.00, 91.17, 90.00

Refinement procedure

Resolution	43.798 - 1.829
R-factor	0.1705
Rwork	0.169
R-free	0.19040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hqr
RMSD bond length	0.005
RMSD bond angle	0.711
Data reduction software	HKL-2000 (706E)
Data scaling software	HKL-2000 (706E)
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.798	1.900
High resolution limit [Å]	1.829	1.829
Rmerge	0.101	0.908
Number of reflections	38965
<I/σ(I)>	11.3375	2
Completeness [%]	99.7	100
Redundancy	3.6	3.7
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.2 M ammonium chloride, 20 % w/v polyethylene glycol 3350