5L9V

HIF prolyl hydroxylase 2 (PHD2-R281C/P317C) cross-linked to HIF-1alpha NODD-L397C/D412C and N-oxalylglycine (NOG) (complex-1)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Egl nine homolog 1	polymer	252	27930.7	2	UniProt (Q9GZT9) Pfam (PF13640)	Homo sapiens (Human)	Hypoxia-inducible factor prolyl hydroxylase 2,HPH-2,Prolyl hydroxylase domain-containing protein 2,PHD2,SM-20
2	C, D (C, D)	Hypoxia-inducible factor 1-alpha	polymer	19	1895.2	2	UniProt (Q16665)	Homo sapiens	HIF1-alpha,ARNT-interacting protein,Basic-helix-loop-helix-PAS protein MOP1,Class E basic helix-loop-helix protein 78,bHLHe78,Member of PAS protein 1,PAS domain-containing protein 8
3	E, G (A, B)	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
4	F, H (A, B)	N-OXALYLGLYCINE	non-polymer		147.1	2	Chemie (OGA)
5	I, J, K, L (A, B, C, D)	water	water		18.0	224	Chemie (HOH)

Sequence modifications

A, B: 181 - 426 (UniProt: Q9GZT9)

PDB	External Database	Details
Gly 175	-	expression tag
Ser 176	-	expression tag
His 177	-	expression tag
Met 178	-	expression tag
Ala 179	-	expression tag
Ser 180	-	expression tag
Ala 201	Cys 201	engineered mutation
Cys 281	Arg 281	engineered mutation
Cys 317	Pro 317	engineered mutation
Ala 398	Arg 398	engineered mutation

C, D: 395 - 413 (UniProt: Q16665)

PDB	External Database	Details
Cys 397	Leu 397	engineered mutation
Cys 412	Asp 412	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	59651.9
Non-Polymers*	Number of molecules	4
	Total formula weight	404.0
All*	Total formula weight	60055.9

*Water molecules are not included.