5L8H
Structure of USP46-UbVME
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-30 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.93 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 91.950, 104.660, 135.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.410 - 1.850 |
R-factor | 0.16757 |
Rwork | 0.166 |
R-free | 0.19411 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ibi |
RMSD bond length | 0.017 |
RMSD bond angle | 1.758 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.410 | 1.890 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.120 | 0.484 |
Number of reflections | 55291 | |
<I/σ(I)> | 17.68 | 2 |
Completeness [%] | 98.8 | 99.7 |
Redundancy | 3.56 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.96M Sodium Citrate pH7.5 and 0.1mM zinc chloride Cryo - 20% Glycerol |