5L6D
Crystal structure of the human METTL3-METTL14 complex bound to SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-14 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.03965 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 63.822, 63.822, 225.626 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.634 - 1.852 |
| R-factor | 0.1588 |
| Rwork | 0.157 |
| R-free | 0.19160 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.112 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.650 | 1.960 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.071 | 0.678 |
| Number of reflections | 46603 | |
| <I/σ(I)> | 34.19 | 4.91 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 19.5 | 19.3 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 22% PEG 3350, 300 mM Mg acetate |
