5L11
Human Liver Receptor Homologue-1 (LRH-1) Bound to RJW100 and a Fragment of TIF-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2014-10-10 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 46.418, 46.418, 220.259 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.491 - 1.849 |
R-factor | 0.2011 |
Rwork | 0.198 |
R-free | 0.24130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3plz |
RMSD bond length | 0.004 |
RMSD bond angle | 0.578 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.920 |
High resolution limit [Å] | 1.849 | 3.990 | 1.850 |
Rmerge | 0.094 | 0.069 | 0.582 |
Number of reflections | 21130 | ||
<I/σ(I)> | 10.6 | ||
Completeness [%] | 96.8 | 99.2 | 86.1 |
Redundancy | 8.9 | 10.5 | 6.8 |
CC(1/2) | 0.997 | 0.878 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.05 M Sodium Acetate, pH 4.6, PEG 4000 5-11%, glycerol 5-10% |