5L0N
PKG I's Carboxyl Terminal Cyclic Nucleotide Binding Domain (CNB-B) in a complex with RP-cGMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97741 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.889, 54.305, 56.450 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.577 - 1.285 |
R-factor | 0.1678 |
Rwork | 0.167 |
R-free | 0.18210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ku8 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.966 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.880 | 1.310 |
High resolution limit [Å] | 1.280 | 1.290 |
Rmerge | 0.050 | 0.470 |
Number of reflections | 31866 | |
<I/σ(I)> | 18.4 | 2.9 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 8 | 7 |
CC(1/2) | 0.999 | 0.915 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 277 | 0.8 M Lithium sulfate monohydrate. 0.1 M Sodium acetate trihydrate pH 4.0, 4% v/v Polyethylene glycol 200 |