5L0I
Human metavinculin MVt R975W cardiomyopathy-associated mutant (residues 959-1134)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.074, 41.519, 44.148 |
| Unit cell angles | 90.00, 96.14, 90.00 |
Refinement procedure
| Resolution | 44.780 - 2.450 |
| R-factor | 0.1969 |
| Rwork | 0.195 |
| R-free | 0.23820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3myi |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.780 | 2.550 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rpim | 0.023 | |
| Number of reflections | 5966 | |
| <I/σ(I)> | 49.2 | 37 |
| Completeness [%] | 98.0 | 99 |
| Redundancy | 6.6 | 6.9 |
| CC(1/2) | 0.998 | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 294 | 0.1 M Na-citrate tribasic dihydrate, 2% Tacsimate (pH 5), and 16% PEG 3350 |
