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5L0G

Human metavinculin MVt Q971R, R975D, T978R mutant (residues 959-1134) in complex with PIP2

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 22-ID
Synchrotron siteAPS
Beamline22-ID
Temperature [K]100
Detector technologyCCD
Collection date2015-10-09
DetectorRAYONIX MX300-HS
Wavelength(s)1.0
Spacegroup nameP 32
Unit cell lengths56.858, 56.858, 193.132
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution49.240 - 3.400
R-factor0.1841
Rwork0.180
R-free0.22060
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3myi
RMSD bond length0.010
RMSD bond angle1.140
Data reduction softwareXDS
Data scaling softwareSCALA
Phasing softwareMOLREP
Refinement softwareBUSTER (2.10.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]96.5703.730
High resolution limit [Å]3.4003.400
Rpim0.103
Number of reflections9632
<I/σ(I)>8.53.6
Completeness [%]100.0100
Redundancy11.111.2
CC(1/2)0.9830.906
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP729312% PEG 3350, 2% tryptone, and 0.05 M HEPES

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