5L0G
Human metavinculin MVt Q971R, R975D, T978R mutant (residues 959-1134) in complex with PIP2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-09 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 |
Unit cell lengths | 56.858, 56.858, 193.132 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.240 - 3.400 |
R-factor | 0.1841 |
Rwork | 0.180 |
R-free | 0.22060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3myi |
RMSD bond length | 0.010 |
RMSD bond angle | 1.140 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 96.570 | 3.730 |
High resolution limit [Å] | 3.400 | 3.400 |
Rpim | 0.103 | |
Number of reflections | 9632 | |
<I/σ(I)> | 8.5 | 3.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 11.1 | 11.2 |
CC(1/2) | 0.983 | 0.906 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 12% PEG 3350, 2% tryptone, and 0.05 M HEPES |