5L03
Crystal structure of 2-C-METHYL-D-ERYTHRITOL 2,4-CYCLODIPHOSPHATE Synthase from BURKHOLDERIA PSEUDOMALLEI bound to L-tryptophan hydroxamate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2015-03-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.515, 68.126, 60.659 |
| Unit cell angles | 90.00, 96.93, 90.00 |
Refinement procedure
| Resolution | 30.714 - 1.469 |
| R-factor | 0.1798 |
| Rwork | 0.179 |
| R-free | 0.19950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.919 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | PHENIX (1.10_2142) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.721 |
| High resolution limit [Å] | 1.469 |
| Number of reflections | 79230 |
| <I/σ(I)> | 19.9 |
| Completeness [%] | 99.0 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 292 | Tris,NaCl, PEG 4000, ZnCl2 |
