5KYC
Crystal structure of USP7 catalytic domain [V302K] mutant in complex with ubiquitin (malonate bound)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 116.330, 43.540, 84.760 |
Unit cell angles | 90.00, 107.55, 90.00 |
Refinement procedure
Resolution | 40.529 - 1.426 |
R-factor | 0.1707 |
Rwork | 0.170 |
R-free | 0.19160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nb8 1ubq |
RMSD bond length | 0.009 |
RMSD bond angle | 0.966 |
Data reduction software | MOSFLM (7.1.1) |
Data scaling software | Aimless (0.2.8) |
Phasing software | PHASER (1.10.1) |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.530 | 40.530 | 1.450 |
High resolution limit [Å] | 1.426 | 7.680 | 1.430 |
Rmerge | 0.073 | 0.052 | 0.384 |
Number of reflections | 75388 | ||
<I/σ(I)> | 8.3 | ||
Completeness [%] | 99.5 | 99.6 | 99.2 |
Redundancy | 3 | 3.3 | 2.7 |
CC(1/2) | 0.994 | 0.993 | 0.799 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 292 | 8% tacsimate pH 4.0, 20% PEG3350 |