5KUE
Human SeMet incorporated I141M/L146M mitochondrial calcium uniporter (residues 72-189) crystal structure with magnesium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-18 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.979 |
Spacegroup name | P 65 |
Unit cell lengths | 55.500, 55.500, 69.020 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.443 - 1.500 |
R-factor | 0.2017 |
Rwork | 0.201 |
R-free | 0.21240 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.398 |
Data reduction software | iMOSFLM |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.060 |
High resolution limit [Å] | 1.500 |
Rmerge | 0.094 |
Number of reflections | 19352 |
<I/σ(I)> | 15.1 |
Completeness [%] | 100.0 |
Redundancy | 11.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.32 M magnesium chloride, 0.1 M Tris pH 8.5, 24 % PEG 400, 1.6 M ammonium sulfate |