5KT5
Teranry complex of human DNA polymerase iota R96G inserting dCMPNPP opposite template G in the presence of Mn2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-19 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 98.111, 98.111, 202.761 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.483 - 2.798 |
R-factor | 0.2258 |
Rwork | 0.224 |
R-free | 0.26640 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.629 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.483 | 50.000 | 2.850 |
High resolution limit [Å] | 2.798 | 7.590 | 2.800 |
Rmerge | 0.117 | 0.047 | 0.842 |
Number of reflections | 14993 | ||
<I/σ(I)> | 6 | ||
Completeness [%] | 99.9 | 99.3 | 99.9 |
Redundancy | 21 | 17.5 | 21.3 |
CC(1/2) | 0.999 | 0.959 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 18% PEG MME 5000, 0.1M MES buffer, 0.3M ammonium sulfate |