5KRB
GCNF DNA Binding Domain - Oct4 DR0 Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2016-01-06 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.649, 69.469, 84.536 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.944 - 2.101 |
R-factor | 0.2183 |
Rwork | 0.216 |
R-free | 0.26830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Previous low resolution structure |
RMSD bond length | 0.003 |
RMSD bond angle | 0.565 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
Rmerge | 0.152 | 0.127 | 0.656 |
Rmeas | 0.167 | 0.140 | 0.758 |
Rpim | 0.069 | 0.057 | 0.371 |
Total number of observations | 95317 | ||
Number of reflections | 18322 | ||
<I/σ(I)> | 8.9 | ||
Completeness [%] | 96.2 | 99.4 | 80.2 |
Redundancy | 5.2 | 6.2 | 3.5 |
CC(1/2) | 0.978 | 0.690 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1M Tris pH 8.5, 20% PEG 3350, 3% glycerol |