5KRB

GCNF DNA Binding Domain - Oct4 DR0 Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	80
Detector technology	CCD
Collection date	2016-01-06
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	53.649, 69.469, 84.536
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.944 - 2.101
R-factor	0.2183
Rwork	0.216
R-free	0.26830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Previous low resolution structure
RMSD bond length	0.003
RMSD bond angle	0.565
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.152	0.127	0.656
Rmeas	0.167	0.140	0.758
Rpim	0.069	0.057	0.371
Total number of observations	95317
Number of reflections	18322
<I/σ(I)>	8.9
Completeness [%]	96.2	99.4	80.2
Redundancy	5.2	6.2	3.5
CC(1/2)		0.978	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1M Tris pH 8.5, 20% PEG 3350, 3% glycerol