5KR7
KDM4C bound to pyrazolo-pyrimidine scaffold
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-04 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 89.817, 89.876, 100.011 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 66.820 - 1.900 |
R-factor | 0.1945 |
Rwork | 0.192 |
R-free | 0.23390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dxu |
RMSD bond length | 0.012 |
RMSD bond angle | 1.467 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 66.820 |
High resolution limit [Å] | 1.900 |
Number of reflections | 64012 |
<I/σ(I)> | 13.7 |
Completeness [%] | 99.2 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 10-15% PEG 3350 0.2 magnesium chloride 0.1 HEPES pH 7.5 |