5KQV
Insulin receptor ectodomain construct comprising domains L1,CR,L2, FnIII-1 and alphaCT peptide in complex with bovine insulin and FAB 83-14 (REVISED STRUCTURE)
Replaces: 3W14Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.953691 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 118.150, 140.100, 190.022 |
| Unit cell angles | 90.00, 95.04, 90.00 |
Refinement procedure
| Resolution | 42.490 - 4.400 |
| R-factor | 0.2307 |
| Rwork | 0.229 |
| R-free | 0.26630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zxb |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.370 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.500 |
| High resolution limit [Å] | 4.400 | 4.400 |
| Rmerge | 0.206 | 1.310 |
| Number of reflections | 17344 | 1064 |
| <I/σ(I)> | 2.77 | 0.78 |
| Completeness [%] | 87.7 | 84.9 |
| Redundancy | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 9% POLYETHYLENE GLYCOL 3350, 200MM PROLINE, 100MM HEPES/NAOH, PH 7.5 |
