5KEN
EBOV GP in complex with variable Fab domains of IgGs c4G7 and c13C6
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.3 |
| Software | DoG Picker,Coot,Rosetta,RELION,Leginon,CTFFIND3,UCSF Chimera,MODELLER |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
