5KEG
Crystal structure of APOBEC3A in complex with a single-stranded DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-02-04 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.54 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 56.583, 72.666, 114.971 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.710 - 2.200 |
Rwork | 0.177 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xxo |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.710 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.091 | 0.528 |
Number of reflections | 12162 | |
<I/σ(I)> | 27.9 | 3 |
Completeness [%] | 98.2 | 82.9 |
Redundancy | 13.1 | 8.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100 mM MOPS 50 mM MgCl2 50 mM CaCl2 23% PEG 3350 15% 2-methyl-2,4-pentanediol |