5KEG

Crystal structure of APOBEC3A in complex with a single-stranded DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-04
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54
Spacegroup name	I 2 2 2
Unit cell lengths	56.583, 72.666, 114.971
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.710 - 2.200
Rwork	0.177
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xxo
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.710	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.091	0.528
Number of reflections	12162
<I/σ(I)>	27.9	3
Completeness [%]	98.2	82.9
Redundancy	13.1	8.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	100 mM MOPS 50 mM MgCl2 50 mM CaCl2 23% PEG 3350 15% 2-methyl-2,4-pentanediol