5K9I
Crystal structure of c-SRC in complex with a covalent lysine probe
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-02-06 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.012, 63.306, 73.770 |
| Unit cell angles | 78.85, 88.75, 89.93 |
Refinement procedure
| Resolution | 28.924 - 2.500 |
| R-factor | 0.1933 |
| Rwork | 0.191 |
| R-free | 0.24870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.242 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 72.360 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 25009 |
| <I/σ(I)> | 16.41 |
| Completeness [%] | 97.0 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 293 | 100 mM MES, pH 6.1, 50 mMNaOAc, 16% glycerol and 4% PEG 4000 |
