5K8V

Crystal Structure of Mus musculus Protein Arginine Methyltransferase 4 (CARM1 130-487) with CP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9801
Spacegroup name	P 21 21 2
Unit cell lengths	75.084, 98.094, 206.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.716 - 2.250
R-factor	0.1945
Rwork	0.192
R-free	0.23480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ih3
RMSD bond length	0.002
RMSD bond angle	0.477
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((dev_2386: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.720	2.300
High resolution limit [Å]	2.250	2.250
Rmerge	0.282	2.132
Number of reflections	73068
<I/σ(I)>	5.7	1.1
Completeness [%]	99.8	97.6
Redundancy	6.5	6.6
CC(1/2)	0.986

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	Tris-HCl pH 8.0 100 mM, PEG 2000 MME 15 %, NaCl 100 mM