5K8D
Crystal structure of rFVIIIFc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 136.295, 136.295, 365.098 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.600 - 4.190 |
| R-factor | 0.3011 |
| Rwork | 0.298 |
| R-free | 0.36190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2r7e |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.896 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.5.2) |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 4.270 |
| High resolution limit [Å] | 4.200 | 11.360 | 4.200 |
| Rmerge | 0.174 | 0.045 | |
| Rmeas | 0.181 | 0.047 | |
| Rpim | 0.054 | 0.013 | 0.471 |
| Total number of observations | 360235 | ||
| Number of reflections | 26141 | ||
| <I/σ(I)> | 4.7 | ||
| Completeness [%] | 99.8 | 96 | 100 |
| Redundancy | 13.8 | 12.3 | 12.6 |
| CC(1/2) | 0.999 | 0.855 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.3 | 297 | 5% Ethanol, 100mM TRIS pH 7.3 |
