5K8D

Crystal structure of rFVIIIFc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-01
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9792
Spacegroup name	P 41 21 2
Unit cell lengths	136.295, 136.295, 365.098
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.600 - 4.190
R-factor	0.3011
Rwork	0.298
R-free	0.36190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2r7e
RMSD bond length	0.016
RMSD bond angle	1.896
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER (2.5.2)
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	4.270
High resolution limit [Å]	4.200	11.360	4.200
Rmerge	0.174	0.045
Rmeas	0.181	0.047
Rpim	0.054	0.013	0.471
Total number of observations	360235
Number of reflections	26141
<I/σ(I)>	4.7
Completeness [%]	99.8	96	100
Redundancy	13.8	12.3	12.6
CC(1/2)		0.999	0.855

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.3	297	5% Ethanol, 100mM TRIS pH 7.3