5K77
Dbr1 in complex with 7-mer branched RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-06 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97950 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.151, 141.892, 212.511 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 118.005 - 2.170 |
R-factor | 0.1839 |
Rwork | 0.182 |
R-free | 0.22290 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4pef |
RMSD bond length | 0.002 |
RMSD bond angle | 0.484 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 118.005 | 2.290 |
High resolution limit [Å] | 2.170 | 2.170 |
Rmerge | 0.118 | 0.720 |
Number of reflections | 220944 | |
<I/σ(I)> | 10.5 | 1.4 |
Completeness [%] | 99.7 | 99.8 |
Redundancy | 4.6 | 3.8 |
CC(1/2) | 0.984 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | PEG 3350, lithium sulfate, glycerol, bis-tris |