5K65

Crystal structure of VEGF binding IgG1-Fc (Fcab CT6)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-05
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9677
Spacegroup name	P 2 21 21
Unit cell lengths	58.490, 72.000, 168.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.028 - 2.500
R-factor	0.223
Rwork	0.221
R-free	0.24630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5k64
RMSD bond length	0.003
RMSD bond angle	0.633
Data reduction software	XDS (v.Oct-2015)
Data scaling software	XDS (v.Oct-2015)
Phasing software	PHENIX (1.10-2247)
Refinement software	PHENIX (1.10-2247)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.028	2.589
High resolution limit [Å]	2.500	2.500
Rmerge	0.125	1.871
Number of reflections	25171
<I/σ(I)>	7.69	0.74
Completeness [%]	99.0	100
Redundancy	4.4	4.7
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	Morpheus_C6 0.03M of each NPS: (sodium nitrate, disodium hydrogen phosphate, ammonium sulfate). 0.1M MOPS/HEPES-Na pH 7.5 10% w/v PEG 8000, 20% v/v ethylene glycol