5K5L
Homo sapiens CCCTC-binding factor (CTCF) ZnF6-8 and H19 sequence DNA complex structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-26 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.2829 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 126.792, 52.397, 69.135 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.725 - 3.125 |
| R-factor | 0.2379 |
| Rwork | 0.236 |
| R-free | 0.27020 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.535 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((dev_2313: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.725 | 3.210 |
| High resolution limit [Å] | 3.125 | 3.125 |
| Rmerge | 0.114 | 0.392 |
| Number of reflections | 15139 | |
| <I/σ(I)> | 14.917 | 3.556 |
| Completeness [%] | 98.7 | 91.1 |
| Redundancy | 7.5 | 4.8 |
| CC(1/2) | 0.980 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 289 | 20% (w/v) polyethylene glycol 8,000 0.1 M CHES-NaOH pH 9.5 |
