5K5H
Homo sapiens CCCTC-binding factor (CTCF) ZnF4-7 and DNA complex structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-26 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.27046 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 52.800, 52.800, 166.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.321 - 3.108 |
| R-factor | 0.2395 |
| Rwork | 0.236 |
| R-free | 0.26350 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.454 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((dev_2400: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.321 | 3.190 |
| High resolution limit [Å] | 3.108 | 3.108 |
| Rmerge | 0.088 | 0.059 |
| Number of reflections | 44172 | |
| <I/σ(I)> | 17.56 | |
| Completeness [%] | 70.8 | 4.7 |
| Redundancy | 7.7 | 7.9 |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 25% (w/v) polyethylene glycol 3350, 0.2 M ammonium acetate, 0.1 M bis-tris HCl, pH 5.5 |
