5K3Y
Crystal structure of AuroraB/INCENP in complex with BI 811283
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2005-07-21 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.907, 67.295, 116.618 |
| Unit cell angles | 90.00, 96.19, 90.00 |
Refinement procedure
| Resolution | 32.000 - 1.600 |
| R-factor | 0.181 |
| Rwork | 0.180 |
| R-free | 0.20100 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.880 |
| Data scaling software | SCALA (3.2.5) |
| Phasing software | MOLREP |
| Refinement software | BUSTER-TNT (2.11.6) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.151 | 45.644 | 1.580 |
| High resolution limit [Å] | 1.497 | 4.740 | 1.500 |
| Rmerge | 0.115 | 0.062 | 0.607 |
| Rmeas | 0.138 | ||
| Total number of observations | 288940 | ||
| Number of reflections | 109777 | ||
| <I/σ(I)> | 8 | 8.9 | 1.1 |
| Completeness [%] | 97.2 | 81.5 | 92 |
| Redundancy | 2.6 | 2.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 296 | 22% PEG 3350, 0.1M Bis-Tris propane pH 8.0, 2 mM TCEP |
