5K24
Crystal structure of the complex between phosphatase PRL-2 in the oxidized state with the Bateman domain of murine magnesium transporter CNNM3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-20 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9773 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 154.222, 52.119, 102.855 |
| Unit cell angles | 90.00, 106.02, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.100 |
| R-factor | 0.25534 |
| Rwork | 0.252 |
| R-free | 0.30327 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.837 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.150 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.078 | 0.644 |
| Number of reflections | 13689 | |
| <I/σ(I)> | 33.9 | 2.8 |
| Completeness [%] | 98.9 | 100 |
| Redundancy | 4.6 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.2 M ammonium citrate, 19% PEG 3350, 10 mM L-cysteine |
