5K1A
Crystal structure of the UAF1-USP12 complex in C2 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 262.425, 103.301, 178.466 |
| Unit cell angles | 90.00, 117.47, 90.00 |
Refinement procedure
| Resolution | 49.104 - 2.300 |
| R-factor | 0.1901 |
| Rwork | 0.188 |
| R-free | 0.23940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5K16 and 5K1B |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.158 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1760) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.091 | 0.574 |
| Number of reflections | 187452 | |
| <I/σ(I)> | 19 | 1.7 |
| Completeness [%] | 99.8 | 98.9 |
| Redundancy | 3.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 0.1 M sodium citrate, 7% PEG 3350, 0.15 M NaCl |
