5JWE
Crystal structure of H-2Db in complex with the LCMV-derived GP92-101 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-01 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.934 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 85.500, 176.300, 85.600 |
Unit cell angles | 90.00, 119.80, 90.00 |
Refinement procedure
Resolution | 19.450 - 2.400 |
R-factor | 0.2024 |
Rwork | 0.199 |
R-free | 0.25890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1n5a |
RMSD bond length | 0.010 |
RMSD bond angle | 1.241 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.610 | 2.430 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.070 | 0.690 |
Number of reflections | 84550 | |
<I/σ(I)> | 12.7 | 1.8 |
Completeness [%] | 94.2 | 91 |
Redundancy | 3.4 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 100 mM Ammonium Sulfate, 100 mM Tris-HCl, 25% PEG 6000 |