5JW4

Structure of MEDI8852 Fab Fragment in Complex with H5 HA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	151.260, 386.360, 165.860
Unit cell angles	90.00, 90.44, 90.00

Refinement procedure

Resolution	29.796 - 3.700
R-factor	0.2741
Rwork	0.274
R-free	0.28510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4BGW and 5JW5
RMSD bond length	0.018
RMSD bond angle	1.470
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.796		3.800
High resolution limit [Å]	3.700	16.550	3.700
Rmerge	0.123	0.019	0.912
Number of reflections	98353
<I/σ(I)>	7.05	36.27	0.99
Completeness [%]	97.4	74.5	98.3
Redundancy	2.18
CC(1/2)	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	16% PEG3350, 0.1 M PIPES pH 7.0, cryoprotected with 25% ethylene glycol