5JUW
complex of Dot1l with SS148
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792912 |
| Spacegroup name | P 65 |
| Unit cell lengths | 153.258, 153.258, 50.723 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.000 - 2.280 |
| R-factor | 0.1899 |
| Rwork | 0.189 |
| R-free | 0.21530 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3uwp |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.483 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.8) |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.380 | 47.380 | 2.360 |
| High resolution limit [Å] | 2.280 | 8.830 | 2.280 |
| Rmerge | 0.090 | 0.026 | 0.977 |
| Rmeas | 0.027 | 1.018 | |
| Rpim | 0.008 | 0.287 | |
| Total number of observations | 6420 | 38340 | |
| Number of reflections | 31441 | ||
| <I/σ(I)> | 23.2 | 70.4 | 3.1 |
| Completeness [%] | 100.0 | 99.2 | 100 |
| Redundancy | 12.4 | 10.9 | 12.5 |
| CC(1/2) | 0.999 | 1.000 | 0.856 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 1.5 M ammonium sulfate, 0.1 M ammonium acetate |
