5JS2
Human Argonaute-2 Bound to a Modified siRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2014-07-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979450 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.102, 107.552, 68.893 |
| Unit cell angles | 90.00, 107.39, 90.00 |
Refinement procedure
| Resolution | 38.983 - 2.954 |
| R-factor | 0.2243 |
| Rwork | 0.222 |
| R-free | 0.26110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ola |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.183 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.983 | 3.130 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.098 | 0.608 |
| Number of reflections | 18175 | |
| <I/σ(I)> | 10.2 | 1.8 |
| Completeness [%] | 98.3 | 95.7 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 12% PEG 3350, 75mM Phenol, 12% Isopropanol, 100mM Tris |
