5JQS
Crystal structure of deubiquitinase MINDY-1 in complex with Ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-10 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97623 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 82.336, 82.336, 332.464 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.630 - 2.650 |
| R-factor | 0.2067 |
| Rwork | 0.205 |
| R-free | 0.23090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jkn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.104 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.630 | 2.780 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.089 | 0.595 |
| Number of reflections | 20518 | |
| <I/σ(I)> | 17.1 | 3.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.4 | 10 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293.15 | 100mM MES, 10% Dioxane and 1.6 M ammonium sulphate |
