5JQE

Crystal structure of caspase8 tDED

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	110
Detector technology	CCD
Collection date	2014-05-06
Detector	ADSC QUANTUM 315
Wavelength(s)	0.987
Spacegroup name	P 64
Unit cell lengths	130.923, 130.923, 67.956
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.532 - 3.157
R-factor	0.219
Rwork	0.216
R-free	0.27380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	MBP
RMSD bond length	0.007
RMSD bond angle	1.033
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.150
Number of reflections	11622
<I/σ(I)>	8
Completeness [%]	100.0
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8	293	0.1M MMT buffer, pH 8.0, 25% PEG 1500