5JQ6
Crystal structure of ClfA in complex with the Fab fragment of Tefibazumab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2007-03-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 234.287, 84.086, 48.626 |
| Unit cell angles | 90.00, 98.90, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.21042 |
| Rwork | 0.208 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vr3 1f8t |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.257 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.090 |
| Number of reflections | 36373 |
| <I/σ(I)> | 7.3 |
| Completeness [%] | 100.0 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | PEG 4000, isopropanol, 0.1 M Hepes |
