5JLE
Crystal structure of SETD2 bound to SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9952 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.540, 77.152, 78.693 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.884 - 2.400 |
| R-factor | 0.2117 |
| Rwork | 0.208 |
| R-free | 0.25040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4h12 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.876 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 6.510 | 2.400 |
| Rmerge | 0.081 | 0.052 | 0.841 |
| Total number of observations | 90045 | ||
| Number of reflections | 12885 | ||
| <I/σ(I)> | 11.6 | ||
| Completeness [%] | 99.4 | 92.3 | 100 |
| Redundancy | 7 | 5.8 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.1 M KSCN, 30% PEG2000mme |
