5JBG

Crystal structure of chicken LGP2 with 5'ppp 26-mer hairpin RNA with 3' GG overhang and ADP-AlF4-Mg2+ at 2.0 A resolution.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.072
Spacegroup name	P 21 21 21
Unit cell lengths	69.860, 97.430, 122.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.270 - 2.000
R-factor	0.21945
Rwork	0.218
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jb2
RMSD bond length	0.008
RMSD bond angle	1.278
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.270	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.259	0.795
Number of reflections	56661
<I/σ(I)>	6.31	1.38
Completeness [%]	99.9	98.7
Redundancy	4.47	4.35

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	277	chLGP2, directly after size exclusion chromatography, was mixed with dsRNA in a 1:1 ratio and incubated for 30 minutes on ice. The complex was concentrated with an Amicon Ultra concentrator to around 10 mg/ml and then 2 mM ADP:AlF4 and 2 mM MgCl2 were added. 5 prime triphosphate, 3 prime GG overhang 26-mer hairpin RNA:ADP:AlF4 complex was mixed with reservoir buffer containing 0.1 M citric acid pH 5.0, 0.8 M ammonium sulphate in a 1:1 ratio.