5J6S
Crystal structure of Endoplasmic Reticulum Aminopeptidase 2 (ERAP2) in complex with a hydroxamic derivative ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.540, 134.330, 127.580 |
| Unit cell angles | 90.00, 91.71, 90.00 |
Refinement procedure
| Resolution | 73.507 - 2.800 |
| R-factor | 0.2085 |
| Rwork | 0.205 |
| R-free | 0.27560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ab0 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.387 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.510 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.112 | 1.110 |
| Number of reflections | 60772 | |
| <I/σ(I)> | 8.6 | 1.7 |
| Completeness [%] | 99.6 | 99.4 |
| Redundancy | 5.1 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.3 | 277 | 7 %(w/v) PEG 8000, 20 %(v/v) ethylene glycol, 59 mM MES, 41 mM imidazole |
