5J0A
Crystal structure of PDZ-binding kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.558, 97.905, 162.835 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.760 - 2.740 |
R-factor | 0.222 |
Rwork | 0.222 |
R-free | 0.27100 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.760 | 2.870 |
High resolution limit [Å] | 2.740 | 2.740 |
Number of reflections | 32541 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M lithium sulfate, 2.0 M ammonium sulfate, 0.1 M CAPS |